BiacoreTM comparability tool for quantitating binding similarities in IgG Fcγ receptor analysis

Rapid screening of IgG quality attributes – effects on Fc receptor binding

The interactions of therapeutic antibodies with fragment crystallizable γ (Fcγ) receptors and neonatal Fc receptors (FcRn) are measured in vitro as indicators of antibody functional performance. Antibodies are anchored to immune cells through the Fc tail, and these interactions are important for the efficacy and safety of therapeutic antibodies. High-throughput binding studies on each of the hu...

Recognition of IgG by Fcγ Receptor: The Role of Fc Glycosylation and The binding of peptide inhibitors

Human Cytomegalovirus Fcγ Binding Proteins gp34 and gp68 Antagonize Fcγ Receptors I, II and III

Human cytomegalovirus (HCMV) establishes lifelong infection with recurrent episodes of virus production and shedding despite the presence of adaptive immunological memory responses including HCMV immune immunoglobulin G (IgG). Very little is known how HCMV evades from humoral and cellular IgG-dependent immune responses, the latter being executed by cells expressing surface receptors for the Fc ...

Fcγ RIIB activation leads to inhibition of signalling by independently ligated receptors

The inhibitory IgG receptor, Fcγ RIIB, blocks signalling by co-aggregated antigen receptors on mature and activated B-cells. Fcγ RIIB is also expressed by immature B-cells; however, its function on these cells has not been defined. In the present paper, we demonstrate that immature B-cells are highly sensitive to inhibitory signalling mediated by Fcγ RIIB. Co-aggregation of Fcγ RIIB with the B-...

Effects of anchor structure and glycosylation of Fcγ receptor III on ligand binding affinity

Isoforms of the Fcγ receptor III (FcγRIII or CD16) are cell surface receptors for the Fc portion of IgG and important regulators of humoral immune responses. Different ligand binding kinetics of FcγRIII isoforms are obtained in three dimensions by surface plasmon resonance and in two dimensions by a micropipette adhesion frequency assay. We show that the anchor structure of CD16 isoforms isolat...